Now once the sequence run has been done on the 3730 with your Sanger
sequences, you need to process them and
to look for a singular nucleotide polymorphisms.
So, in my sequence, you can see in this region right
here a black peak, a green peak, and a blue peak.
That's my sequence.
And then in, in this individual, it's a black peak, green peak, blue peak.
But in this individual, it's a black peak, green peak, red
peak.
This particular, sequence right here, where it's a c in,
in my sequence, a c in this other reference sequence.
And a t in this bottom sequence.
What this means is that we have a polymorphism in this position right here.
That is what we call a single nucleotide polymorphism.
And these single nucleotide polymorphisms, or snips, are what
we use in the genetic technology and the genetic techniques
that we described in lecture.
These chromatographs are relatively hard to look at,
so what the computer also does, the programs
also do, is they transform this peak information
into actual DNA sequence information, into the g's
t's a's and c's, so that this region right here that my finger is pointing to,
is the same region we talked about when
we were looking at the pigs in the chromatagraph.
So my sequence has a c. The middle reference sequence
has a c and the bottom reference sequence
has a t indicating a single nucleotide polymorphism which
is the main thing that you use in
doing genomic analysis that we talked about in lecture.