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Avis et commentaires pour l'étudiant pour Basics of Extracellular Vesicles par Université de Californie à Irvine

4.7
137 notes
32 Avis

À propos du cours

This course aims to provide the basic knowledge about extracellular vesicles (EV) a generic term including exosomes, microvesicles, microparticles, ectosomes, oncosomes, prostasomes, and many others. It covers areas such as EV history, nomenclature, biogenesis, EV cargo as well as the release and uptake mechanisms, collection and processing prior to isolation, different isolation methods, characterization and quantification techniques. This course is divided into five modules. Module 1 is an introduction to the field and will cover the nomenclature and the history of EVs. Module 2 will focus on the biogenesis, release and uptake mechanisms of EVs as well as the different EV cargos (RNA, protein, lipids). In Module 3, we will focus on the collection and processing of cell culture media and body fluids such as blood, breast milk, cerebrospinal fluid and urine prior to isolation of EVs. Module 4 and 5 will present different isolation methods and characterization/quantification techniques, respectively. Here differential ultracentrifugation, size exclusion chromatography, density gradient, kit based precipitation, electron microscopy (EM), cryo-TEM, flow cytometry, atomic-force microscopy and nanoparticle tracking analysis will be presented. The recommended prerequisites are college-freshman-level biology and biochemistry. After a completed course you should be able to: + Discuss the nomenclature and subgroups of extracellular vesicles. + Describe the RNA, protein and lipid content of extracellular vesicles. + Describe the basic concepts about the most common isolation and characterization techniques and how these techniques are used in the EV field. + State the benefits and limitations of the most common isolation and characterization techniques for extracellular vesicles. + Explain the considerations that are important during the collection and isolation of EVs from different body fluid. + Describe the release and uptake mechanisms of extracellular vesicles All lectures are given in English. Each of the five modules will be followed by an exam. All exams will be in the format of multiple choice questions. The course is organized in collaboration between the International Society for Extracellular Vesicles (ISEV), University of California Irvine (USA), University of Gothenburg (Sweden) and Pohang University of Science and Technology (South Korea)....

Meilleurs avis

MH

Jan 11, 2019

Awosome course. Lot of information given with paper based data and suggestion. I really love it. I am also working with Exosome. I use cell culture media from PC12 and fibroblast cell line.

MV

Jan 31, 2018

The only problem I faced, has to do with the subtitles. They have lots of mismatches and mistakes. Nevertheless, the MOOC is undoubtedly great and pretty educational.

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1 - 25 sur 29 Examens pour Basics of Extracellular Vesicles

By Sandra M D A

Mar 09, 2019

Me ha gustado mucho este curso, ya que he aprendido muchos conocimientos sobre las vesículas extracelulares.

By Esteban J O H

May 15, 2019

Very complete introduction to the field of exosomes with relevant bibliography and relevant papers to move deep into this amiazing and exciting field. Thanks for all the person who made this possible and hope to see you on the next ISEV2020.

By MD N H

Jan 11, 2019

Awosome course. Lot of information given with paper based data and suggestion. I really love it. I am also working with Exosome. I use cell culture media from PC12 and fibroblast cell line.

By Karem J N

Aug 20, 2018

El curso es muy completo en cuanto a conceptos generales de los exosomas y a las tecnicas generales que para analisis y caracterizacion

By Azalea K

Jul 27, 2018

Very good and informative course. I learnt a lot about EVs and exosomes, which are usually not found in published articles or books. I wish somebody had recommended this course before I started my PhD, would have saved me a lot of time!!

By lcy9086

Apr 01, 2018

I am sorry to say that many parts of the lecture provided by the Korean professor is inaudible......SO you can only learn from his slides :(

By David d V L

Mar 29, 2018

Excellents classes I have learned a lot of procotols about EVs

By Shalmali P

Mar 03, 2018

Very nice nd inormative presentations. But few words in many presentations are not audible.

By Minos V

Jan 31, 2018

The only problem I faced, has to do with the subtitles. They have lots of mismatches and mistakes. Nevertheless, the MOOC is undoubtedly great and pretty educational.

By Judith U D M

Jan 01, 2018

I loved this course! I currently work in the liposome field and I was curious about learning EVs especially because its a young and growing field where they will be a lot of things to study. I liked the combination of scientists who got on board to share their knowledge in the field and I will definitely think of studying EVs in the future.

By Kaio M R

Oct 10, 2017

ÓTIMO

By Xiaokun L

Jul 20, 2017

nice

By Ashley R

Jun 30, 2017

I learned SO much from this course. I'm so glad I took it! Although I did the free (audit) version, I do wish that I could have taken all of the quizzes for each week to ensure I fully internalized and understood the information.

By Lorena L R V

May 29, 2017

Excelente curso, reafirma los conceptos básicos, da consejos para las distintas técnicas.

By Enrique V O

May 11, 2017

It is good much info about vesicles that you can't get from the books and techniques to study them.

By Wenhao L

Apr 26, 2017

Very wonderful courses! Better than reading 100 papers!

By Janviere K

Apr 24, 2017

I enjoyed how the course touched on all important aspects of EVs in depth providing relevant and supportive literature

By Denise S

Mar 16, 2017

It's a great course, learnt a lot. It would have been great if the slides were provided too and addition of discussion forums would also help to cultivate what we have learnt better by exchanging views with fellow classmates. Thanks very much for providing the course.

By pbmansfield

Mar 06, 2017

Well put together and very informative. Great job!

By Shiyou C

Feb 13, 2017

The course provide a general introduction of this field and the methods usde in the experiments.

By Mohammad R

Feb 05, 2017

This course gives an excellent overview of the field!

By Jai M

Jan 02, 2017

I am a masters student doing an EV related lab project, this course has provided great insight into EV research from leaders in the field. Importantly, this has made me aware of what to look out for in the literature.

Many Thanks!

By Sherri C

Dec 12, 2016

Excellent course. A great overview plus a lot of detailed information that can't be found by reading the literature.

By Shukhman B

Nov 12, 2016

The most important benefit of this course is the fact that it exists. At present the course is mediocre to bad: its educational value is low: transcripts are disgusting, they are not proofread, omitted pats of sentences, inconsistent English, they almost completely obscure, disrupt the sense of the presentation. The course offers no slides. I see obvious negligence in preparation of many lectures (especially bad week three).

The target audience for the course is professional biologist that are comparable with arithmetic and elementary algebra and can easily use an internet browser.

The course contains numerous mistakes: in particular, the elementary formula Dr. Cecilia Lasser presented in her week 4 lecture on Differential Ultracentrifugation contains an error.

The lectures presented by non-native English speakers are very difficult to understand because of poor & inconsistent English.

Please, make the lecture slide available for your students.

Please, arrange a forum giving a possibility to ask a questions and get understanding of the material that remains unclear.

The lectures on electron microscopy were very good, - thank you, Johanna!

The lectures on AFM was the only one that was just excellent, - Thank you, Dr. Shivani Sharma.

Please, review the sequence of lectures: I think the week 5 material may be moved to week 3.

By Adel D

Nov 03, 2016

very good!