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Basics of Extracellular Vesicles, Université de Californie à Irvine

4.7
134 notes
31 avis

À propos de ce cours

This course aims to provide the basic knowledge about extracellular vesicles (EV) a generic term including exosomes, microvesicles, microparticles, ectosomes, oncosomes, prostasomes, and many others. It covers areas such as EV history, nomenclature, biogenesis, EV cargo as well as the release and uptake mechanisms, collection and processing prior to isolation, different isolation methods, characterization and quantification techniques. This course is divided into five modules. Module 1 is an introduction to the field and will cover the nomenclature and the history of EVs. Module 2 will focus on the biogenesis, release and uptake mechanisms of EVs as well as the different EV cargos (RNA, protein, lipids). In Module 3, we will focus on the collection and processing of cell culture media and body fluids such as blood, breast milk, cerebrospinal fluid and urine prior to isolation of EVs. Module 4 and 5 will present different isolation methods and characterization/quantification techniques, respectively. Here differential ultracentrifugation, size exclusion chromatography, density gradient, kit based precipitation, electron microscopy (EM), cryo-TEM, flow cytometry, atomic-force microscopy and nanoparticle tracking analysis will be presented. The recommended prerequisites are college-freshman-level biology and biochemistry. After a completed course you should be able to: + Discuss the nomenclature and subgroups of extracellular vesicles. + Describe the RNA, protein and lipid content of extracellular vesicles. + Describe the basic concepts about the most common isolation and characterization techniques and how these techniques are used in the EV field. + State the benefits and limitations of the most common isolation and characterization techniques for extracellular vesicles. + Explain the considerations that are important during the collection and isolation of EVs from different body fluid. + Describe the release and uptake mechanisms of extracellular vesicles All lectures are given in English. Each of the five modules will be followed by an exam. All exams will be in the format of multiple choice questions. The course is organized in collaboration between the International Society for Extracellular Vesicles (ISEV), University of California Irvine (USA), University of Gothenburg (Sweden) and Pohang University of Science and Technology (South Korea)....

Meilleurs avis

par MH

Jan 11, 2019

Awosome course. Lot of information given with paper based data and suggestion. I really love it. I am also working with Exosome. I use cell culture media from PC12 and fibroblast cell line.

par MV

Jan 31, 2018

The only problem I faced, has to do with the subtitles. They have lots of mismatches and mistakes. Nevertheless, the MOOC is undoubtedly great and pretty educational.

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28 avis

par Sandra María Delgado Argudo

Mar 09, 2019

Me ha gustado mucho este curso, ya que he aprendido muchos conocimientos sobre las vesículas extracelulares.

par MD Nurul Huda

Jan 11, 2019

Awosome course. Lot of information given with paper based data and suggestion. I really love it. I am also working with Exosome. I use cell culture media from PC12 and fibroblast cell line.

par Karem J Noris-Suárez

Aug 20, 2018

El curso es muy completo en cuanto a conceptos generales de los exosomas y a las tecnicas generales que para analisis y caracterizacion

par Azalea Khan

Jul 27, 2018

Very good and informative course. I learnt a lot about EVs and exosomes, which are usually not found in published articles or books. I wish somebody had recommended this course before I started my PhD, would have saved me a lot of time!!

par lcy9086

Apr 01, 2018

I am sorry to say that many parts of the lecture provided by the Korean professor is inaudible......SO you can only learn from his slides :(

par David del Valle Laveaga

Mar 29, 2018

Excellents classes I have learned a lot of procotols about EVs

par Shalmali Pendse

Mar 03, 2018

Very nice nd inormative presentations. But few words in many presentations are not audible.

par Minos Vandoulakis

Jan 31, 2018

The only problem I faced, has to do with the subtitles. They have lots of mismatches and mistakes. Nevertheless, the MOOC is undoubtedly great and pretty educational.

par Judith Upeka De Mel

Jan 01, 2018

I loved this course! I currently work in the liposome field and I was curious about learning EVs especially because its a young and growing field where they will be a lot of things to study. I liked the combination of scientists who got on board to share their knowledge in the field and I will definitely think of studying EVs in the future.

par Kaio Mota Ribeiro

Oct 10, 2017

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