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Avis et commentaires pour d'étudiants pour Getting started in cryo-EM par Caltech

264 évaluations
51 avis

À propos du cours

This class covers the fundamental principles underlying cryo-electron microscopy (cryo-EM) starting with the basic anatomy of electron microscopes, an introduction to Fourier transforms, and the principles of image formation. Building upon that foundation, the class then covers the sample preparation issues, data collection strategies, and basic image processing workflows for all 3 basic modalities of modern cryo-EM: tomography, single particle analysis, and 2-D crystallography. Philosophy: The course emphasizes concepts rather than mathematical details, taught through numerous drawings and example images. It is meant for anyone interested in the burgeoning fields of cryo-EM and 3-D EM, including cell biologists or molecular biologists without extensive training in mathematics or imaging physics and practicing electron microscopists who want to broaden their understanding of the field. The class is perfect as a primer for anyone who is about to be trained as a cryo-electron microscopist, or for anyone who needs an introduction to the field to be able to understand the literature or the talks and conversations they will hear at cryo-EM meetings. Pre-requisites: The recommended prerequisites are college-freshman-level math, physics, and biochemistry. Pace: There are 14.5 hours of lecture videos total separated into 40 individual “modules” lasting on average 20 minutes each. Each module has at the end a list of “concept check” questions you can use to test your knowledge of what was presented. As the modules are grouped into seven major subjects, one reasonable plan would be to go through one major subject each day. That would mean watching a couple hours of lecture and spending another hour or so thinking through the concept check questions each day for a week. Another reasonable plan would be to go through one module each day for a little over a month, or even three modules a week (Monday, Wednesday, and Friday) for a 3-month term. It is likely that as you then move on to actually begin using a cryo-EM or otherwise engage in the field, you will want to repeat certain modules....

Meilleurs avis


13 avr. 2022

I am about to start a PhD in Structural Biology and this course gave me really good ropes on how a a microscope is composed and how an image is formed. One of the best courses I have evere taken!


18 févr. 2018

Great course. I thought it was presented in a very informative way and i learned a lot about cryo-EM. In fact, it made me want to learn more about it. Thank you.

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26 - 50 sur 51 Avis pour Getting started in cryo-EM

par meera k m

22 févr. 2016

very informative, simple and easily understandable. Love it

par sreenu b

11 août 2016

Excellent class and Presentation with all clarity concepts

par Daniil B

14 nov. 2015

Very nice description of electronic microscopy techniques

par Igor N

9 juin 2016

Really good introduction to cryo-EM and EM in general!

par Deleted A

9 mars 2021

Precise, in-depth and full of valuable knowledge.

par Vadim S

23 juin 2017

Great job! Clear explanations, drawings, schemes.

par Nivetha R

12 oct. 2019

Extremely Helpful and very comfortable to learn

par Xiaolong C

23 déc. 2015

very great course, including so much details.

par Bing W

18 mai 2016

It's really useful. Thanks so much.

par 赵炜

25 oct. 2015

The best EM course available online

par ANIL K S

12 sept. 2018

very good for the beginner like me

par Baharak M

27 déc. 2015

It" Awesome. Thank you.

par Chao-Han Y

28 oct. 2015


par Francois T

17 août 2016

Thank you Dr. Jensen!

par 董炜哲

4 sept. 2015

absolutely fantastic!

par Janusz B

11 avr. 2020

Fantastic lectures!

par jadson c d s

27 déc. 2015


par Shokir P

13 sept. 2015

great course!

par stanwang

18 oct. 2017


par Scott C

29 nov. 2015

Very good

par minagquan

9 sept. 2015


par Herman A

1 févr. 2016

Great information, great teacher, greater visual presentation. Disclaimer: I only took the first week.

par Marcus G

1 janv. 2016

Great introduction to Cryo-EM for biologists that covers all the basics in a clear and concise manner.

par Haris F

11 janv. 2016

Pioneering online course in Electron Microscopy.

par Christine L

18 oct. 2016

Excellent content