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Programme du cours : ce que vous apprendrez dans ce cours

Semaine
1
1 heure pour terminer

Welcome

Welcome to the course!

...
3 vidéos (Total 19 min), 3 lectures
3 vidéos
Description of Parts7 min
Introduction: Why Electrons?8 min
3 lectures
Welcome from Professor Jensen10 min
About the Instructor10 min
Concept Check Questions: Why Electrons?10 min
Semaine
2
3 heures pour terminer

Currents, Coils, Knobs and Names: Basic anatomy of the electron microscope

...
9 vidéos (Total 94 min), 8 lectures
9 vidéos
Electron Guns18 min
EM Lenses
The Column26 min
Sample Chamber14 min
Energy Filters7 min
Detectors11 min
Vacuum Systems9 min
Summary and Safety5 min
8 lectures
Concept Check Questions: Electron Guns10 min
Concept Check Questions: Electron Lenses10 min
Concept Check Questions: The Column10 min
Concept Check Questions: The Sample Chamber10 min
Concept Check Questions: Energy Filters10 min
Concept Check Questions: Electron Detectors10 min
Concept Check Questions: Vacuum Systems10 min
Concept Check Questions: Summary and Safety10 min
Semaine
3
3 heures pour terminer

Fourier Transforms and Reciprocal Space for Beginners

...
7 vidéos (Total 134 min), 6 lectures
7 vidéos
1-D Sine Waves and their Sums32 min
1-D Reciprocal Space20 min
2-D Waves and Images19 min
2-D Transforms and Filters32 min
3-D Waves and Transforms13 min
Convolution and Cross-Correlation15 min
6 lectures
Concept Check Questions: 1-D Sine Waves and Their Sums10 min
Concept Check Questions: 1-D Reciprocal Space10 min
Concept Check Questions: 2-D Waves and Images10 min
Concept Check Questions: 2-D Transforms and Filters10 min
Concept Check Questions: 3-D Waves and Transforms10 min
Concept Check Questions: Convolution and Cross-correlation10 min
Semaine
4
4 heures pour terminer

Image Formation

...
7 vidéos (Total 164 min), 6 lectures
7 vidéos
Amplitude and Phase Contrast30 min
Wave Propagation and Phase Shifts30 min
The Contrast Transfer Function33 min
Defocus and its Effects21 min
Envelopes16 min
CTF Correction31 min
6 lectures
Concept Check Questions: Amplitude and Phase Contrast10 min
Concept Check Questions: Wave Propagation and Phase Shifts10 min
Concept Check Questions: The Contrast Transfer Function10 min
Concept Check Questions: Defocus and its Effects10 min
Concept Check Questions: Envelopes10 min
Concept Check Questions: CTF Correction10 min
Semaine
5
2 heures pour terminer

Fundamental Challenges in Biological EM

...
6 vidéos (Total 96 min), 5 lectures
6 vidéos
Sample Prep - Room Temperature Methods20 min
Sample Prep Methods Involving Freezing27 min
Sample Prep Grids11 min
3-D Reconstruction11 min
Dose Limitations22 min
5 lectures
Concept Check Questions: Sample Prep - Room Temperature Methods10 min
Concept Check Questions: Sample Prep - Methods Involving Freezing10 min
Concept Check Questions: Sample Prep - Grids10 min
Concept Check Questions: 3-D Reconstruction10 min
Concept Check Questions: Dose Limitations10 min
Semaine
6
4 heures pour terminer

Single Particle Analysis

...
5 vidéos (Total 159 min), 6 lectures
5 vidéos
Data collection (imaging)21 min
Reconstruction basics31 min
Reconstruction: additional topics44 min
Interpretation and limitations38 min
6 lectures
Concept check questions: Introduction to single particle analysis10 min
Concept Check Questions: Special sample prep issues10 min
Concept Check Questions: Data collection (imaging)10 min
Concept Check Questions: Reconstruction basics10 min
Concept Check Questions: Reconstruction - additional topics10 min
Concept Check Questions: Interpretation and limitations10 min
Semaine
7
3 heures pour terminer

Tomography

...
5 vidéos (Total 131 min), 5 lectures
5 vidéos
Tomography Data Collection and Reconstruction40 min
Tomography Identifying Objects of Interest24 min
Tomography Limitations29 min
Tomography Parameters and Handedness12 min
5 lectures
Concept Check Questions: Introduction: Tomography10 min
Concept Check Questions: Tomography Data Collection and Reconstruction10 min
Concept Check Questions: Identifying Objects in Tomograms10 min
Concept Check Questions: Tomography Limitations10 min
Concept Check Questions: Tomography Parameters and Handedness10 min
Semaine
8
2 heures pour terminer

2-D Crystallography

...
5 vidéos (Total 55 min), 4 lectures
5 vidéos
FT of a 2-D Crystal24 min
2-D Crystallography Data Collection and Reconstruction17 min
Helical Tubes3 min
Farewell2 min
4 lectures
Concept Check Questions: Introduction: 2-D Crystallography and Sample Prep10 min
Concept Check Questions: Fourier Transform of a 2-D Crystal10 min
Concept Check Questions: Data Collection and Reconstruction10 min
Concept Check Questions: Helical Tubes10 min
4.9
42 avisChevron Right

Principaux examens pour Getting started in cryo-EM

par LRFeb 19th 2018

Great course. I thought it was presented in a very informative way and i learned a lot about cryo-EM. In fact, it made me want to learn more about it. Thank you.

par ALJul 20th 2016

An excellent introduction to the topic, explaining from the basics to the detail of things. Useful and recommended to anyone in structural biology.

Enseignant

Avatar

Grant J. Jensen

Professor of Biophysics and Biology; Investigator, Howard Hughes Medical Institute
Biology and Biological Engineering

À propos de Caltech

Caltech is a world-renowned science and engineering research and education institution, where extraordinary faculty and students seek answers to complex questions, discover new knowledge, lead innovation, and transform our future. Caltech's mission is to expand human knowledge and benefit society through research integrated with education. We investigate the most challenging, fundamental problems in science and technology in a singularly collegial, interdisciplinary atmosphere, while educating outstanding students to become creative members of society....

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